dc.contributor.author | Coskun Z.M. | |
dc.contributor.author | Ersoz M. | |
dc.contributor.author | Adas M. | |
dc.contributor.author | Hancer V.S. | |
dc.contributor.author | Boysan S.N. | |
dc.contributor.author | Gonen M.S. | |
dc.contributor.author | Acar A. | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T15:52:45Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T15:52:45Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0188-4409 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.arcmed.2019.05.012 | |
dc.identifier.uri | http://hdl.handle.net/11446/1708 | |
dc.description.abstract | Background/Aim: Diabetic nephropathy (DN) is one of the most serious microvascular complications in diabetic patients. The kruppel-like transcription factor-4 (KLF-4) affects the expression of genes involved in the pathogenesis of DN. The present study aims to identify the KLF-4 expression and DNA methylation (DNAMe) status in patients with type-2 diabetes (T2D) and DN and to reveal the contribution of the KLF-4 to the development of DN. Material and Methods: The cohort study was performed with blood samples from 120 individuals; T2D group (n = 40), DN group (n = 40) and control group (n = 40). The expression level of the KLF-4 gene was analyzed using the real-time polymerase chain reaction (qRT-PCR) and the methylation profile detected using the methylation-specific PCR (MS-PCR) technique. Results: According to our findings, KLF-4 mRNA expression in the T2D group was 1.60 fold lower than in the control group (p = 0.001). In the DN group, the expression of KLF-4 mRNA was 2.92-fold less than that of the T2D group (p = 0.001). There was no significant alteration in the DNAMe status among the groups. Conclusion: Our findings showed that regardless of the DNAMe status, KLF-4 gene expression may play a role in the development of T2D and DN. This suggests that the KLF-4 gene may be the target gene in understanding the mechanism of nephropathy, which is the most important complication of diabetes, and planning nephropathy-related treatments, but the data should be supported with more studies. © 2019 IMSS | en_US |
dc.description.sponsorship | Firat University Scientific Research Projects Management Unit | en_US |
dc.description.sponsorship | This study was supported by the Scientific Research Projects Coordination Unit of Istanbul Bilim University . Project no: 2015017 . | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Inc. | en_US |
dc.identifier.doi | 10.1016/j.arcmed.2019.05.012 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Diabetic nephropathy | en_US |
dc.subject | DNA methylation | en_US |
dc.subject | Epigenetics | en_US |
dc.subject | Kruppel-like transcription factor-4 | en_US |
dc.subject | Type 2 diabetes | en_US |
dc.title | Kruppel-Like Transcription Factor-4 Gene Expression and DNA Methylation Status in Type 2 Diabetes and Diabetic Nephropathy Patients | en_US |
dc.type | article | en_US |
dc.relation.journal | Archives of Medical Research | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.volume | 50 | en_US |
dc.identifier.startpage | 91 | en_US |
dc.identifier.endpage | 97 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | DBÜ | en_US |