Standard cremaster muscle model for ischemia reperfusion

Abstract

Ischemia — reperfusion injury (IRI) is a serious complication of revascularisation that biases a result of many surgical procedures. The pathophysiology of IRI is very complex and still not fully understood. For years a laboratory model of muscle ischemia reperfusion have been developed to provide reliable scientific tool for observation of IRI and investigation of possible therapeutic strategies. Cremaster muscle model of IRI plays an important role in research of IRI due to its similarity to clinical scenarios of limb ischemia, transplantation, replantation. This model provides unique opportunity to visualize important events that occur during reperfusion like capillary perfusion, leukocyte-endothelial interaction, microvasculature response and endothelial permeability. It is a potent model for intravital investigation of muscle microcirculation subjected to IRI. © Springer-Verlag London 2015.