EFFECTS OF FLUORESCENT MARKED MAACKIA AMURENSIS-LECTIN-1 AND WHEAT GERM AGLUTIN ON THE CELL SURFACE GLYCAN PROFILES IN TWO DIFFERENT BREAST CANCER CELL LINES
Özet
Objective: Many methods such as pathological examinations are used to diagnose breast cancer. In addition to all these tests and methods, the use of glycobiology has also increased significantly in cancer research. Differences in sugar residues observed on the membrane surfaces of breast cancer cell lines ER (+)-creating MCF-7 and ER (-) MDA-MB-231 were specifically marked with FITC-(Maackia Amurensis-Lectin-1) Ve FITC-(Wheat Germ Aglutinin) The intensity of radiation is discussed relatively. The aim of using this method is to clearly reveal that the differences in the sialic acid units in the membranes of breast cancer cell lines can be precisely separated in as little as two hours. Material and Method: MCF-7 and MDA-MB-231) cells were used in our study. [FITC-(Maackia Amurensis-Lectin-1) Ve FITC-(Wheat Germ Aglutin)] were purchased from the respective companies. Following this step, the FITC marked products were prepared and marked according to usage protocols. Results: FITC-labeled Maackia amurensis Lectin-1 was applied to MCF-7 and MDA-MB-231 breast cancer cell lines. Subsequently, the MDA-MB-231 cell line showed a relatively more intense radiation than the MCF-7. The FITC marked Wheat germ agglutinin was applied to the same cancer cell lines. As a result of this marking, radiation of similar intensity was detected in both cancer lines. Conclusion: These differences seen in the membranes of the MCF-7 and MDA-MB-231 cell lines used in the study are significant. Two different types of breast cancer cell types were rapidly separated using the two hour staining method applied to the cells. At the same time, with the help of immunofluorescent-labeled lectins, the availability of glyconoconjugates was demonstrated for rapid and specific discrimination.
