dc.contributor.author | Ersoz, Melike | |
dc.contributor.author | Kacar, Ayse Karatug | |
dc.contributor.author | Sezekler, Isil | |
dc.contributor.author | Coskun, Zeynep Mine | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T15:55:32Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T15:55:32Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 2602-3032 | |
dc.identifier.issn | 2602-3040 | |
dc.identifier.uri | https://dx.doi.org/10.17826/cumj.435450 | |
dc.identifier.uri | http://hdl.handle.net/11446/2052 | |
dc.description | WOS: 000458742100009 | en_US |
dc.description.abstract | Purpose: The study aims to examine the effects of boric acid at different concentrations applied to green tea on heat shock protein (HSP) 70 and HSP 90 in MCF-7 cells. Materials and Methods: Boric acid in sodium tetraborate buffer of 0 mg/m(2) (group 1), 100 mg/m(2) (group 2), 300 mg/m(2) (group 3), and 500 mg/m(2) (group 4) were applied to soil where Camelia sinensis (green tea) grown. The collected green tea leaves were dried and brewed in water at 80 degrees C for 40 minutes. Cytotoxicity was analyzed by MTT method. The prepared green tea extracts (250 mu g/ml) were applied to MCF-7 cells. The mRNA expressions of HSP 70 and HSP 90 were determined by qRT-PCR. The HSP 70 and HSP 90 protein levels were measured by Western blotting method. Results: 100 and 500 mg/m(2) boric-acid-applied green tea extracts increased HSP 90 mRNA expressions in MCF-7 cells compared to green tea extract without boric acid. HSP 70 and HSP 90 protein levels did not show any change between MCF-7 cells with boric-acid-applied green tea extract and green tea extract without boric acid. Conclusion: According to our results, the protein expressions of HSP 70 and HSP 90 in MCF-7 cells changed by neither boric-acid-applied green tea extracts nor green tea extracts without any boric-acid-applied. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | CUKUROVA UNIV, FAC MEDICINE | en_US |
dc.identifier.doi | 10.17826/cumj.435450 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | MCF-7 cells | en_US |
dc.subject | heat shock protein | en_US |
dc.subject | green tea | en_US |
dc.subject | boric acid | en_US |
dc.subject | cytotoxicity | en_US |
dc.title | Effects of boric-acid-applied green tea on the expressions of heat shock proteins in MCF-7 cells | en_US |
dc.type | article | en_US |
dc.relation.journal | CUKUROVA MEDICAL JOURNAL | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.volume | 44 | en_US |
dc.identifier.startpage | 66 | en_US |
dc.identifier.endpage | 71 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Ersoz, Melike -- Coskun, Zeynep Mine] Istanbul Bilim Univ, Fac Sci & Art, Dept Mol Biol & Genet, Istanbul, Turkey -- [Kacar, Ayse Karatug] Istanbul Univ, Fac Sci, Dept Biol, Istanbul, Turkey -- [Sezekler, Isil] Marmara Univ, Fac Sci & Art, Dept Biol, Istanbul, Turkey | en_US |