dc.contributor.author | Sogut, Ibrahim | |
dc.contributor.author | Uysal, Onur | |
dc.contributor.author | Oglakci, Aysegul | |
dc.contributor.author | Yucel, Ferruh | |
dc.contributor.author | Kartkaya, Kazim | |
dc.contributor.author | Kanbak, Gungor | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T15:56:32Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T15:56:32Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0256-7040 | |
dc.identifier.issn | 1433-0350 | |
dc.identifier.uri | https://dx.doi.org/10.1007/s00381-016-3309-6 | |
dc.identifier.uri | http://hdl.handle.net/11446/2327 | |
dc.description | WOS: 000398041500006 | en_US |
dc.description | PubMed ID: 28062893 | en_US |
dc.description.abstract | Purpose Alcohol consumption in pregnancy may cause fetal alcohol syndrome (FAS) in the infant. This study aims to investigate prenatal alcohol exposure related neuroapoptosis on the cerebral cortex tissues of newborn rats and possible neuroprotective effects of betaine, folic acid, and combined therapy. Methods Pregnant rats were divided into five experimental groups: control, ethanol, ethanol + betaine, ethanol + folic acid, and ethanol + betaine + folic acid combined therapy groups. We measured cytochrome c release, caspase-3, calpain and cathepsin B and L. enzyme activities. In order to observe apoptotic cells in the early stages, TUNEL method was chosen together with histologic methods such as assessing the diameters of the apoptotic cells, their distribution in unit volume and volume proportion of cortical intact neuron nuclei. Results Calpain, caspase-3 activities, and cytochrome c levels were significantly increased in alcohol group while cathepsin B and L. activities were also found to be elevated albeit not statistically significant. These increases were significantly reversed by folic acid and betaine + folic acid treatments. While ethanol increased the number of apoptotic cells, this increase was prevented in ethanol + betaine and ethanol + betaine + folic acid groups. Morphometric examination showed that the mean diameter of apoptotic cells was increased with ethanol administration while this increase was reduced by betaine and betaine + folic acid treatments. Conclusion We observed that ethanol is capable of triggering apoptotic cell death in the newborn rat brains. Furthermore, folic acid, betaine, and combined therapy of these supplements may reduce neuroapoptosis related to prenatal alcohol consumption, and might be effective on preventing fetal alcohol syndrome in infants. | en_US |
dc.description.sponsorship | TUBITAK [109S510] | en_US |
dc.description.sponsorship | This work was supported by TUBITAK 109S510 project. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SPRINGER | en_US |
dc.identifier.doi | 10.1007/s00381-016-3309-6 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Betaine | en_US |
dc.subject | Calpain | en_US |
dc.subject | Caspase-3 | en_US |
dc.subject | Cytochrome c | en_US |
dc.subject | Folic acid | en_US |
dc.subject | Neuroapoptosis | en_US |
dc.title | Prenatal alcohol-induced neuroapoptosis in rat brain cerebral cortex: protective effect of folic acid and betaine | en_US |
dc.type | article | en_US |
dc.relation.journal | CHILDS NERVOUS SYSTEM | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.volume | 33 | en_US |
dc.identifier.startpage | 407 | en_US |
dc.identifier.endpage | 417 | en_US |
dc.contributor.authorID | 0000-0001-6800-5607 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Sogut, Ibrahim] Istanbul Bilim Univ, Vocat Sch Hlth Serv, Yazarlar Sok 17, TR-34394 Istanbul, Turkey -- [Uysal, Onur] Eskisehir Osmangazi Univ, Vocat Sch Hlth Serv, TR-26480 Eskisehir, Turkey -- [Oglakci, Aysegul -- Kartkaya, Kazim -- Kanbak, Gungor] Eskisehir Osmangazi Univ, Sch Med, Dept Biochem, TR-26480 Eskisehir, Turkey -- [Yucel, Ferruh] Eskisehir Osmangazi Univ, Sch Med, Dept Anat, TR-26480 Eskisehir, Turkey | en_US |