dc.contributor.author | Altinkilic, Emre Murat | |
dc.contributor.author | Isbir, Selim | |
dc.contributor.author | Gormus, Uzay | |
dc.contributor.author | Yilmaz, Seda Gulec | |
dc.contributor.author | Dalan, Altay Burak | |
dc.contributor.author | Duman, Selvi | |
dc.contributor.author | Isbir, Turgay | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T15:57:02Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T15:57:02Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0258-851X | |
dc.identifier.issn | 1791-7549 | |
dc.identifier.uri | http://hdl.handle.net/11446/2438 | |
dc.description | WOS: 000384933000011 | en_US |
dc.description | PubMed ID: 27566080 | en_US |
dc.description.abstract | Background/Aim: Coronary artery disease (CAD) is a chronic inflammatory disease seen as formation of atherosclerotic plaques (atheroma) in coronary arteries. Recent published papers show that DNA damage and repair mechanisms play a crucial role on the development and severity of atheromas. In this study, we investigated nucleotide excision repair (NER) pathway-related gene polymorphisms in atherosclerosis. XPD, encoded by ERCC2 gene, is an ATP-depended helicase enzyme involved in the NER pathway. Ribonucleotide reductase (RR) is a tetrameric enzyme, synthesizing deoxyribonucleotides from ribonucleotides for DNA synthesis. RR is encoded by the RRM1 and RRM2 genes, which are two subunits of RR enzyme. Materials and Methods: DNA samples isolated from peripheral blood were genotyped with real-time polymerase chain reaction (RT-PCR) for RRM1 (rs12806698), RRM2(rs6859180) and ERCC2 (rs13181) genes. Results: The frequency of the RRM1 AC heterozygote genotype was found to be significantly lower (odds ratio (OR) = 0.369, 95% confidence interval (CI) = 0.179-0.760; p = 0.006), whereas the CC homozygote genotype was found to be significantly higher in patients compared to controls (OR = 7.636, 95% CI = 2.747-21.229; p = 0.000). In addition, the RRM1 A allele was higher in control group (p = 0.000, OR = 0.131 95% CI = 0.047-0.364). For the ERCC2 gene, GG genotype was significantly higher in control group (p = 0.017, OR = 0.387, 95%CI = 0.175-0.152) and TT genotype (p = 0.021) was higher in CAD group. TT genotype had a similar to 3-fold increased risk (OR = 3.615, 95%CI = 1.148-11.380) for CAD. Carrying T allele appears to be a risk factor for CAD (p = 0.017, OR = 2.586, 95%CI = 1.173-5.699), while the G allele might be a risk-reducing factor (p = 0.021, OR = 0.277, 95%CI = 0.088-0.871) for CAD. Conclusion: RRM1 and ERCC gene polymorphisms, having homozygous mutant genotype, might be a risk factor for CAD. RRM1 and ERCC wild type alleles are risk-reducing factor for CAD. Also, carrying RRM1 A allele might have a protective effect for smokers. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | INT INST ANTICANCER RESEARCH | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | RRM1 | en_US |
dc.subject | RRM2 | en_US |
dc.subject | ERCC2 | en_US |
dc.subject | polymorphism | en_US |
dc.subject | CAD | en_US |
dc.title | RRM1, RRM2 and ERCC2 Gene Polymorphisms in Coronary Artery Disease | en_US |
dc.type | article | en_US |
dc.relation.journal | IN VIVO | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.volume | 30 | en_US |
dc.identifier.startpage | 611 | en_US |
dc.identifier.endpage | 615 | en_US |
dc.contributor.authorID | 0000-0002-3124-0184 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Altinkilic, Emre Murat -- Yilmaz, Seda Gulec] Yeditepe Univ, Inst Hlth Sci, Dept Mol Med, Istanbul, Turkey -- [Isbir, Selim -- Duman, Selvi] Marmara Univ, Pendik Educ & Res Hosp, Dept Cardiovasc Surg, Istanbul, Turkey -- [Gormus, Uzay] Istanbul Bilim Univ, Fac Med, Dept Biochem, Istanbul, Turkey -- [Dalan, Altay Burak] Yeditepe Univ, Fac Med, Dept Biochem, Istanbul, Turkey -- [Isbir, Turgay] Yeditepe Univ, Fac Med, Dept Med Biol, Inonu Cad 26 Agustos Yerleskesi, TR-34755 Istanbul, Turkey | en_US |