dc.contributor.author | Cavusoglu, T. | |
dc.contributor.author | Popken, J. | |
dc.contributor.author | Guengoer, T. | |
dc.contributor.author | Yilmaz, O. | |
dc.contributor.author | Uyanikgil, Y. | |
dc.contributor.author | Ates, U. | |
dc.contributor.author | Zakhartchenko, V. | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T15:57:06Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T15:57:06Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0340-2096 | |
dc.identifier.issn | 1439-0264 | |
dc.identifier.uri | https://dx.doi.org/10.1111/ahe.12197 | |
dc.identifier.uri | http://hdl.handle.net/11446/2452 | |
dc.description | WOS: 000379423000005 | en_US |
dc.description | PubMed ID: 26293816 | en_US |
dc.description.abstract | Cryopreservation is the process of freezing and preserving cells and tissues at low temperatures. Controlled slow freezing and vitrification have successfully been used for cryopreservation of mammalian embryos. We investigated the effect of these two cryopreservation methods on in vitro produced four-cell stage bovine embryos which were classified according to their quality and separated into three groups. The first group was maintained as untreated controls (n = 350). Embryos of the second (n = 385) and the third (n = 385) groups were cryopreserved either by controlled slow freezing or by vitrification. Embryos in groups 2 and 3 were thawed after 1 day. Hundred embryos were randomly selected from the control group, and 100 morphologically intact embryos from the second and third group were thawed after 1 day and cultured to observe the development up to the blastocyst stage. The blastocyst development rate was 22% in the control group, 1% in the slow-freezing group and 3% in the vitrification group. Remaining embryos of all three groups were examined by light microscopy, transmission electron microscopy and immunofluorescence confocal microscopy with subsequent histological staining procedures. Cryopreservation caused degenerative changes at the ultra-structural level. Compared with vitrification, slow freezing caused an increased mitochondrial degeneration, cytoplasmic vacuolization, disruption of the nuclear and plasma membrane integrity, organelle disintegration, cytoskeletal damage, a reduced thickness of the zona pellucida and a formation of fractures in the zona pellucida. Further studies are required to understand and decrease the harmful effects of cryopreservation. | en_US |
dc.description.sponsorship | Ege University [2011-Tip-064]; Ege University Local Animal Ethics Committee [2009/25] | en_US |
dc.description.sponsorship | This project was supported by an Ege University Grant (2011-Tip-064), Ege University Local Animal Ethics Committee (Decision Number: 2009/25). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | WILEY | en_US |
dc.identifier.doi | 10.1111/ahe.12197 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.title | Ultra-Structural Alterations in In Vitro Produced Four-Cell Bovine Embryos Following Controlled Slow Freezing or Vitrification | en_US |
dc.type | article | en_US |
dc.relation.journal | ANATOMIA HISTOLOGIA EMBRYOLOGIA | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.volume | 45 | en_US |
dc.identifier.startpage | 291 | en_US |
dc.identifier.endpage | 307 | en_US |
dc.contributor.authorID | 0000-0002-7709-3626 | en_US |
dc.contributor.authorID | 0000-0002-4016-0522 | en_US |
dc.contributor.authorID | 0000-0002-4016-0522 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Cavusoglu, T. -- Yilmaz, O. -- Uyanikgil, Y. -- Baka, M.] Ege Univ, Dept Histol & Embryol, TR-35100 Izmir, Turkey -- [Cavusoglu, T. -- Yilmaz, O. -- Uyanikgil, Y. -- Baka, M.] Ege Univ, Cord Blood Cell Tissue Applicat & Res Ctr, TR-35100 Izmir, Turkey -- [Popken, J.] Univ Munich, Div Anthropol & Human Genet, Bioctr, Grosshadernerstr 2, D-82152 Planegg Martinsried, Germany -- [Guengoer, T. -- Zakhartchenko, V.] Univ Munich, Dept Mol Anim Breeding & Biotechnol, Hackerstr 27, D-85764 Oberschleissheim, Germany -- [Ates, U.] Bilim Univ, Dept Histol & Embryol, Sch Med, TR-34349 Istanbul, Turkey -- [Oztas, E.] Gulhane Mil Med Acad, Dept Histol & Embryol, TR-06010 Ankara, Turkey | en_US |