dc.contributor.author | Ozturk, Tulin | |
dc.contributor.author | Toptas-Hekimoglu, Bahar | |
dc.contributor.author | Eronat, Allison Pinar | |
dc.contributor.author | Saygili, Neslihan | |
dc.contributor.author | Daglar-Aday, Aynur | |
dc.contributor.author | Bassullu, Nuray | |
dc.contributor.author | Isbir, Turgay | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T15:58:10Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T15:58:10Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0258-851X | |
dc.identifier.issn | 1791-7549 | |
dc.identifier.uri | http://hdl.handle.net/11446/2649 | |
dc.description | WOS: 000361049900012 | en_US |
dc.description | PubMed ID: 26359417 | en_US |
dc.description.abstract | Background/Aim: The murine sarcoma viral (V-Raf) oncogene homolog B (BRAF) V600E mutation, which increases protein kinase activity in BRAF-mitogen-activated protein kinase kinase (MEK) - extracellular signal-regulated kinases (ERK) (mitogen-activated protein kinase (MAPK)) signaling, is found in 5-40% of all colorectal carcinoma cases. Proteins with this mutation are reported to be 130-fold more active, which results in induced proliferation, differentiation, cellular survival, and angiogenesis. The aim of the present study was to investigate tumor tissues, together with the surrounding non-tumoral tissues, for BRAF mutation presence, which may be an indicator for possible recurrence or prognosis as in the 'field carcinogenesis' model. Materials and Methods: The BRAF V600E genotype of 152 colorectal adenocarcinoma paraffin-embedded specimens were determined by mutant-allele-specific amplification-polymerase chain reaction. Results: According to our results, the presence of BRAF mutation increases risk of lymph node invasion by 1.55-fold [chi(2)=3.83, p=0.05, odds ratio (OR)=1.55, 95% confidence interval (CI)=1.00-2.42], histologically medium or high-grade tumor by 1.60-fold (chi(2)=4.34, p=0.030, OR= 1.60, 95% CI=1.03-2.48), vascular invasion by 1.55-fold (chi(2)=3.55, p=0.05, OR=1.55, 95% CI=0.99-2.42), perineural invasion by 1.50-fold (chi(2)=3.16, p=0.07, OR=1.5, 95% CI=0.96-2.33) and the combination of these poor prognostic features by 1.54-fold (chi(2)=2.47, p=0.11, OR=1.54, 95% CI=0.93-2.53). We also found that females are more prone to having the mutation and that being female increases the risk of having this mutation by 1.54-fold (chi(2)=3.58, p=0.05, OR=1.54, 95% CI=0.97-2.44). Conclusion: BRAF V600E mutation in non-tumoral surrounding tissue in patients with colorectal cancer may be used as a valuable marker to foresee clinical outcome or a possible recurrence. To our knowledge, this was the first study to take into consideration the non-tumoral surrounding tissues in addition to the tumor tissue. | en_US |
dc.description.sponsorship | Scientific Research Projects Coordination Unit of Istanbul University [11160] | en_US |
dc.description.sponsorship | The Authors thank the Pathology Department of Istanbul University, Cerrahpasa Medical Faculty and Pathology Department, Istanbul Bilim University, Medical Faculty for assistance with sample processing. The Scientific Research Projects Coordination Unit of Istanbul University supported this work. Project No. 11160. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | INT INST ANTICANCER RESEARCH | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | BRAF gene | en_US |
dc.subject | V600E | en_US |
dc.subject | colon cancer | en_US |
dc.subject | mutation | en_US |
dc.subject | field carcinogenesis | en_US |
dc.title | Co-existence of BRAF V600E Gene Mutation in Tumor and Non-tumoral Surrounding Tissues in Colorectal Cancer | en_US |
dc.type | article | en_US |
dc.relation.journal | IN VIVO | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.volume | 29 | en_US |
dc.identifier.startpage | 577 | en_US |
dc.identifier.endpage | 584 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Ozturk, Tulin -- Goksel, Suha] Istanbul Univ, Cerrahpasa Med Fac, Dept Pathol, Istanbul, Turkey -- [Toptas-Hekimoglu, Bahar -- Eronat, Allison Pinar -- Saygili, Neslihan -- Daglar-Aday, Aynur -- Aydogan, Hlya Yilmaz -- Ozturk, Oguz] Istanbul Univ, Dept Mol Med, Expt Med & Res Inst, Istanbul, Turkey -- [Bassullu, Nuray -- Turkmen, Ilknur -- Bulbul, Gulen] Istanbul Bilim Univ, Dept Pathol, Fac Med, Istanbul, Turkey -- [Goksel, Suha] Acibadem Maslak Hosp, Dept Pathol, Istanbul, Turkey -- [Isbir, Turgay] Yeditepe Univ, Dept Med Biol, TR-34755 Istanbul, Turkey | en_US |