dc.contributor.author | Ucar, A. Yaba | |
dc.contributor.author | Kayisli, U. A. | |
dc.contributor.author | Demir, N. | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T16:03:48Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T16:03:48Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1567-2379 | |
dc.identifier.uri | https://dx.doi.org/10.1007/s10735-011-9377-8 | |
dc.identifier.uri | http://hdl.handle.net/11446/3170 | |
dc.description | WOS: 000299376400013 | en_US |
dc.description | PubMed ID: 22143492 | en_US |
dc.description.abstract | The epithelial cells of the mouse endometrium comprises the recurring physiologic changes such as proliferation and apoptosis induced by the reproductive hormones throughout the estrus cycle. The c-Abl is a protein tyrosine kinase, localized in the different cellular compartments such as nucleus, cytoplasm, mitochondria, and endoplasmic reticulum, interacts with different cellular proteins, including signaling adaptors, kinases, phosphatases, cell-cycle regulators, transcription factors and cytoskeletal proteins. Our hypothesis is that the c-Abl expression in the mouse uterine epithelium shows cyclic changes during the estrus cycle that is involved in regulation of the endometrial epithelial cells. The regulation of c-Abl gene and protein expression in the uterus of intact animals in the different cycle phases was investigated using immunohistochemistry, western blot and semi-quantitative RT-PCR. The immunohistochemistry results revealed that the luminal and the glandular epithelium (LE) and (GE), respectively, showed gradually increase in the expression of c-Abl from the proestrus (P) to the metestrus and followed by a decrease in the diestrus (D). c-Abl immunoreactivity was detected in the both LE and GE cells, especially the LE showed diminished the c-Abl protein expression on the D phase and the minimal value was detected on the P day. The c-Abl protein level in the LE was increased during the M, presenting a high correlation with the hormonal level of this cell type. The result of c-Abl RT-PCR analysis was compatible with pattern of c-Abl protein expression. In conclusion, the stage-specific protein pattern of the mammalian c-Abl tyrosine kinase presented a good correlation with the mouse estrus cycle and may have a regulative function during the uterine remodeling. | en_US |
dc.description.sponsorship | Akdeniz University-Antalya/Turkey [2004.02.0122.006] | en_US |
dc.description.sponsorship | This study was supported by the Research Fund of Akdeniz University-Antalya/Turkey (project #2004.02.0122.006). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SPRINGER | en_US |
dc.identifier.doi | 10.1007/s10735-011-9377-8 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | c-Abl | en_US |
dc.subject | Mouse uterus | en_US |
dc.subject | Estrus cycle | en_US |
dc.title | The c-Abl expression in uterine epithelium during the mouse estrus cycle | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF MOLECULAR HISTOLOGY | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.volume | 43 | en_US |
dc.identifier.startpage | 107 | en_US |
dc.identifier.endpage | 113 | en_US |
dc.contributor.authorID | 0000-0003-2865-798X | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Demir, N.] Akdeniz Univ, Fac Med, Dept Histol & Embryol, TR-07070 Antalya, Turkey -- [Ucar, A. Yaba] Istanbul Bilim Univ, Dept Histol & Embryol, Fac Med, TR-34394 Istanbul, Turkey -- [Kayisli, U. A.] Yale Univ, Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT 06520 USA | en_US |