dc.contributor.author | Akin, Demet | |
dc.contributor.author | Ravizza, Teresa | |
dc.contributor.author | Maroso, Mattia | |
dc.contributor.author | Carcak, Nihan | |
dc.contributor.author | Eryigit, Tugba | |
dc.contributor.author | Vanzulli, Ilaria | |
dc.contributor.author | Onat, Filiz Yilmaz | |
dc.date.accessioned | 2019-08-13T12:10:23Z | |
dc.date.accessioned | 2019-08-13T16:03:56Z | |
dc.date.available | 2019-08-13T12:10:23Z | |
dc.date.available | 2019-08-13T16:03:56Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 0969-9961 | |
dc.identifier.issn | 1095-953X | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.nbd.2011.05.015 | |
dc.identifier.uri | http://hdl.handle.net/11446/3197 | |
dc.description | WOS: 000295816200001 | en_US |
dc.description | PubMed ID: 21645619 | en_US |
dc.description.abstract | Interleukin (IL)-1 beta plays a crucial role in the mechanisms of limbic seizures in rodent models of temporal lobe epilepsy. We addressed whether activation of the IL-1 beta signaling occurs in rats with genetic absence epilepsy (GAERS) during the development of spike-and-wave discharges (SWDs). Moreover, we studied whether inhibition of IL-1 beta biosynthesis in GAERS could affect SWD activity. IL-1 beta expression and glia activation were studied by immunocytochemistry in the forebrain of GAERS at postnatal days (PN)14, PN20, and PN90 and in age-matched non-epileptic control Wistar rats. In PN14 GAERS, when no SWDs have developed yet. UT immunostaining was undetectable, and astrocytes and microglia showed a resting phenotype similar to control Wistar rats. In 3 out of 9 PN20 GAERS, IL-1 beta was observed in activated astrocytes of the somatosensory cortex; the cytokine expression was associated with the occurrence of immature-type of SWDs. In all adult PN90 GAERS, when mature SWDs are established, IL-1 beta was observed in reactive astrocytes of the somatosensory cortex but not in adjacent cortical areas or in extra-cortical regions. An age-dependent c-fos activation was found in the somatosensory cortex of GAERS with maximal levels reached in PN90 rats; c-fos was also induced in some thalamic nuclei in PN20 and PN90 GAERS. Inhibition of IL-1 beta biosynthesis in PN90 GAERS by 4-day systemic administration of a specific ICE/Caspase-1 blocker, significantly reduced both SWD number and duration. These results show that IL-1 beta is induced in reactive astrocytes of the somatosensory cortex of GAERS at the onset of SWDs. IL-1 beta has pro-ictogenic properties in this model, and thus it may play a contributing role in the mechanisms underlying the occurrence of absence seizures. (C) 2011 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | Marmara University Scientific Research Committee [SAG-075/120503]; Istanbul Bilim University Scientific Research Committee; EPICURE [LSH-CT-2006-037315]; Fondazione Monzino; NeuroGlia [202167] | en_US |
dc.description.sponsorship | This study was supported by the Marmara University Scientific Research Committee (SAG-075/120503, F.Y.O.), Istanbul Bilim University Scientific Research Committee (D. A.), EPICURE (LSH-CT-2006-037315, A. V.) and Fondazione Monzino (A.V.). Mattia Maroso received a fellowship from NeuroGlia (EU-FP7-project 202167). The authors are grateful to Vertex Pharmaceuticals Incorporated for supplying VX-765 and to Irina Kadiyala (Vertex Pharmaceuticals Incorporated) for her support with the drug formulation. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.identifier.doi | 10.1016/j.nbd.2011.05.015 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Inflammation | en_US |
dc.subject | Absence seizures | en_US |
dc.subject | Interleukins | en_US |
dc.subject | SWD | en_US |
dc.title | IL-1 beta is induced in reactive astrocytes in the somatosensory cortex of rats with genetic absence epilepsy at the onset of spike-and-wave discharges, and contributes to their occurrence | en_US |
dc.type | article | en_US |
dc.relation.journal | NEUROBIOLOGY OF DISEASE | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.volume | 44 | en_US |
dc.identifier.startpage | 259 | en_US |
dc.identifier.endpage | 269 | en_US |
dc.contributor.authorID | 0000-0003-0680-4782 | en_US |
dc.contributor.authorID | 0000-0002-1519-3170 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Ravizza, Teresa -- Maroso, Mattia -- Vanzulli, Ilaria -- Vezzani, Annamaria] Mario Negri Inst Pharmacol Res, Dept Neurosci, I-20156 Milan, Italy -- [Akin, Demet] Istanbul Bilim Univ, Dept Pharmacol, Sch Med, Istanbul, Turkey -- [Carcak, Nihan] Istanbul Univ, Fac Pharm, Dept Pharmacol, Istanbul, Turkey -- [Eryigit, Tugba -- Aker, Rezzan Gulhan -- Onat, Filiz Yilmaz] Marmara Univ, Dept Pharmacol & Clin Pharmacol, Sch Med, Istanbul, Turkey | en_US |