EVALUATION OF OXIDATIVE STRESS IN EXPERIMENTAL ACETAMINOPHEN INDUCED HEPATOTOXICITY: EFFECTS OF DARBEPOETIN ADMINISTRATION

Abstract

Objective Effects of darbepoetin alfa on acetaminophen induced hepatotoxicity have been investigated by evaluating some oxidant/antioxidant parameters and liver histology Material and Method Fifty adult male Sprague Dawley rats were randomly divided into five equal groups Sham group (S) Acetaminophen (800 mg/kg single dose intraperitoneally) injected group (A) Darbepoetin alfa (10 mu g/kg, single dose subcutaneously) administered at the same time with acetaminophen injection group (ASD) before acetaminophen injection darbepoetin administered group (DA) Darbepoetin administered after acetaminophen injection group (AD) Results Plasma AST and ALT levels of the A group the ASD group the DA group and the AD group were significantly higher than the S group (p<0 001) Plasma MDA levels of the ASD group the DA group and the AD group were significantly higher than the A group (p<0 001) Plasma Cu Zn SOD levels of the ASD (p<0 01) DA (p<0 01) and AD (p<0 001) groups was significantly lower than the A group Tissue Cu Zn SOD levels of the ASD (p<0 01) group the DA (p<0 001) group and the AD (p<0 001) groups were significantly lower than the A group Conclusion Darbepoetin alfa administration could not support the antioxidant parameters in acetaminophen induced hepatotoxicity Timing of darbepoetin alfa administration for liver toxicity could be an important factor for the efficacy of the treatment