dc.contributor.author | Ozkaya, Hande Mefkure | |
dc.contributor.author | Sayitoglu, Muge | |
dc.contributor.author | Comunoglu, Nil | |
dc.contributor.author | Sun, Eda | |
dc.contributor.author | Keskin, Fatma Ela | |
dc.contributor.author | Ozata, Duygu | |
dc.contributor.author | Kadioglu, Pinar | |
dc.date.accessioned | 2020-12-02T18:01:22Z | |
dc.date.available | 2020-12-02T18:01:22Z | |
dc.identifier.issn | 0947-7349 | |
dc.identifier.issn | 1439-3646 | |
dc.identifier.uri | https://doi.org/10.1055/a-1274-1330 | |
dc.identifier.uri | http://hdl.handle.net/11446/3592 | |
dc.description | Firtina, Sinem/0000-0002-3370-8545 | en_US |
dc.description | WOS: 000586879900001 | en_US |
dc.description | PubMed: 33091936 | en_US |
dc.description.abstract | Purpose To evaluate the expression of G-protein coupled estrogen receptor (GPER1), aromatase, estrogen receptor alpha (ER alpha), estrogen receptor beta (ER beta), pituitary tumor transforming gene (PTTG), and fibroblast growth factor 2 (FGF2) in GH-secreting and non-functioning adenomas (NFA). Methods Thirty patients with acromegaly and 27 patients with NFA were included. Gene expression was determined via quantitative reverse transcription polymerase chain reaction (QRT-PCR). Protein expression was determined via immunohistochemistry. Results There was no difference, in terms of gene expression of aromatase, ER alpha, PTTG, and FGF2 between the two groups (p> 0.05 for all). ERR gene expression was higher and GPER1 gene expression was lower in GH-secreting adenomas than NFAs (p <0.05 for all). Aromatase and ER beta protein expression was higher in GH-secreting adenomas than NFAs (p = 0.01). None of the tumors expressed ER alpha. GPER1 expression was detected in 62.2 % of the GH-secreting adenomas and 45 % of NFAs. There was no difference in terms of GPER1, PTTG, FGF2 H scores between the two groups (p> 0.05 for all). GPER1 gene expression was positively correlated to ER alpha, ER beta, PTTG, and FGF2 gene expression (p < 0.05 for all). There was a positive correlation between aromatase and GPER1 protein expression (r = 0.31; p = 0.04). Conclusions GPER1 is expressed at both gene and protein level in a substantial portion of GH-secreting adenomas and NFAs. the finding of a positive correlation between GPER1 and ER alpha, ER beta, PTTG, and FGF2 gene expression and aromatase and GPER1 protein expression suggests GPER1 along with aromatase and classical ERs might mediate the effects of estrogen through upregulation of PTTG and FGF2. | en_US |
dc.description.sponsorship | Research Fund of the Istanbul University, Istanbul, Turkey [46991] | en_US |
dc.description.sponsorship | The study was supported by the Research Fund of the Istanbul University, Istanbul, Turkey, project number 46991. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh | en_US |
dc.identifier.doi | 10.1055/a-1274-1330 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | acromegaly | en_US |
dc.subject | aromatase | en_US |
dc.subject | estrogens | en_US |
dc.subject | hormones | en_US |
dc.subject | GPER1 | en_US |
dc.subject | pituitary tumor | en_US |
dc.title | G-protein Coupled Estrogen Receptor Expression in Growth Hormone Secreting and Non-Functioning Adenomas | en_US |
dc.type | article | en_US |
dc.relation.journal | Experimental and Clinical Endocrinology & Diabetes | en_US |
dc.department | DBÜ | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Ozkaya, Hande Mefkure; Kadioglu, Pinar] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Endocrinol & Metab, TR-34303 Istanbul, Turkey; [Sayitoglu, Muge; Sun, Eda; Khodzaev, Khusan; Firtina, Sinem] Istanbul Univ, Aziz Sancar Inst Expt Med, Istanbul, Turkey; [Comunoglu, Nil; Oz, Buge] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Pathol, Istanbul, Turkey; [Keskin, Fatma Ela] Demiroglu Bilim Univ, Dept Endocrinol & Metab, Istanbul, Turkey; [Ozata, Duygu; Hocaoglu, Rabia Hacer] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Internal Med, Istanbul, Turkey; [Tanriover, Necmettin] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Neurosurg, Istanbul, Turkey; [Gazioglu, Nurperi] Demiroglu Bilim Univ, Dept Neurosurg, Istanbul, Turkey; [Tanriover, Necmettin; Kadioglu, Pinar] Istanbul Univ Cerrahpasa, Pituitary Ctr, Istanbul, Turkey | en_US |