dc.contributor.author | Metin-Armagan, Derya | |
dc.contributor.author | Comunoglu, Nil | |
dc.contributor.author | Bulut, Gulay | |
dc.contributor.author | Kadioglu, Pinar | |
dc.contributor.author | Kameda, Hiraku | |
dc.contributor.author | Gazioglu, Nurperi | |
dc.contributor.author | Ozturk, Melek | |
dc.date.accessioned | 2020-12-02T18:01:35Z | |
dc.date.available | 2020-12-02T18:01:35Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1046-3976 | |
dc.identifier.issn | 1559-0097 | |
dc.identifier.uri | https://doi.org/10.1007/s12022-019-09598-x | |
dc.identifier.uri | http://hdl.handle.net/11446/3663 | |
dc.description | Tanriover, Necmettin/0000-0001-7628-9443; Kadioglu, Pinar/0000-0002-8329-140X; comunoglu, nil/0000-0002-2319-1757 | en_US |
dc.description | WOS: 000519375300002 | en_US |
dc.description | PubMed: 31828584 | en_US |
dc.description.abstract | The molecular mechanisms underlying the formation of nonfunctioning pituitary adenomas (NFAs) are largely unknown. in this study, we aimed to understand the relationship between NFAs and functional pituitary adenomas and the possible role of proteins involved in cell cycle, senescence, and DNA damage control mechanisms in the etiology of NFA. We analyzed pATM-S1981, pRb-S608, Rb, pE2F1-S364, p16, E2F1, p73, cyclin D1, and CHEK2 protein expression (in a group of 20 patients with acromegaly, 18 patients with Cushing's disease (CD), and 29 NFA patients) by immunohistochemistry and their relevant mRNA expression by qRT-PCR (in a group of 7 patients with acromegaly, 7 patients with CD, and 7 NFA patients). the clinical and histopathological results on the patients were statistically evaluated. pE2F1-S364 protein expression in the CD group was significantly lower than that in the NFA and acromegaly groups (p = 0.025, p = 0.034, respectively). However, the expression of the p16 protein was lower than in the NFA group than in the CD and acromegaly groups (p = 0.030, p = 0.033, respectively), and E2F1 protein expression was significantly higher in the NFA group than in the CD group (p = 0.025). p73 protein expression in patients with acromegaly was significantly higher (p = 0.031) than that in the CD group. CHEK2 mRNA expression in the CD group was significantly higher than that in the acromegaly group (p = 0.012). the selective and tumor-specific associations between E2F1, pE2F1-S364, CHEK2, and p73 mRNA and protein levels indicate their involvement in pituitary adenoma formation in NFA, CD, and acromegaly patients. | en_US |
dc.description.sponsorship | Research Fund of the Istanbul UniversityIstanbul University [2017-25404] Funding Source: Medline | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Humana Press Inc | en_US |
dc.identifier.doi | 10.1007/s12022-019-09598-x | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Nonfunctioning pituitary adenomas | en_US |
dc.subject | CHEK2 | en_US |
dc.subject | p73 | en_US |
dc.subject | RB | en_US |
dc.subject | p16 | en_US |
dc.title | A Novel Expression Profile of Cell Cycle and DNA Repair Proteins in Nonfunctioning Pituitary Adenomas | en_US |
dc.type | article | en_US |
dc.relation.journal | Endocrine Pathology | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.volume | 31 | en_US |
dc.identifier.startpage | 2 | en_US |
dc.identifier.endpage | 13 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Metin-Armagan, Derya; Ozturk, Melek] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Med Biol, Istanbul, Turkey; [Comunoglu, Nil] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Pathol, Istanbul, Turkey; [Bulut, Gulay] Bahcesehir Univ, Fac Engn & Nat Sci, Dept Mol Biol & Genet, Istanbul, Turkey; [Kadioglu, Pinar] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Endocrinol & Metab, Istanbul, Turkey; [Kameda, Hiraku] Hokkaido Univ, Fac Med, Dept Rheumatol Endocrinol & Nephrol, Sapporo, Hokkaido, Japan; [Kameda, Hiraku] Hokkaido Univ, Grad Sch Med, Sapporo, Hokkaido, Japan; [Gazioglu, Nurperi] Istanbul Bilim Univ, Dept Neurosurg, Istanbul, Turkey; [Tanriover, Necmettin] Istanbul Univ Cerrahpasa, Cerrahpasa Med Sch, Dept Neurosurg, Istanbul, Turkey | en_US |