Ülkemizdeki hepatit D virus genotipi

Abstract

Bu çalışmanın amacı; hepatitis D virusu ile infekte kronik hepatitli hastalarda genotip tayini yapmaktır. Çalışma kapsamına 25 hasta alınmıştır. HDV-RNA Boom metodu, hepatitis D virusu genotip tayini ise RT-PCR ve RFLP metodu ile çalışılmıştır. 22 hastada genotip I saptanmıştır. 3 hastada genotip tayini yapılamamıştır. Sonuç olarak; ülkemizdeki hepatitis D virusu, genotip I’dir.

The aim of this study was to identify hepatitis D virus genotypes in our chronic hepatitis D patients. Twenty-five patients with hepatitis D virus infection were enrolled in this study. HDV-RNA was extracted from the serum by Boom methods. Hepatitis D virus genotype was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) methods. Genotype I was found in 22 patients, and could not be determined in the remaining 3 patients. In conclusion, genotype I is the dominant hepatitis D virus genotype in our country.