dc.contributor.author | Bahar-Tokman, H. | |
dc.contributor.author | Demirci, M. | |
dc.contributor.author | Keskin, F.E. | |
dc.contributor.author | Cagatay, P. | |
dc.contributor.author | Taner, Z. | |
dc.contributor.author | Ozturk-Bakar, Y. | |
dc.contributor.author | Kocazeybek, B.S. | |
dc.date.accessioned | 2022-11-04T19:55:50Z | |
dc.date.available | 2022-11-04T19:55:50Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 1433-6510 | |
dc.identifier.uri | https://doi.org/10.7754/Clin.Lab.2022.211244 | |
dc.identifier.uri | http://hdl.handle.net/11446/4642 | |
dc.description.abstract | Background: The aim of this study was to determine the Firmicutes/Bacteroidetes ratio in the gut microbiota and IL-1β, IL-6, IL-8, TLR2, TLR4 and TLR5 gene expression levels in the blood of adult type 2 diabetes (T2D) patients and compare it with that of adult nondiabetic healthy controls (HC). Methods: Between May 2016 and April 2017, 99 T2D patients and 99 HCs were enrolled in the study. Bacteroidetes and Firmicutes levels were assessed from stool sample DNA and IL-1β, IL-6, IL-8, TLR2, TLR4, and TLR5 gene expression levels assesed from blood sample RNA via qPCR from both T2D patients and healthy controls. Results: The Firmicutes/Bacteroidetes ratio detected in the stool of type 2 diabetes patients was found to be higher with a statistically significant difference (p < 0.0001). Gene expression levels of IL-1β, IL-6, IL-8, TLR2, TLR4, and TLR5 were found to be upregulated. Conclusions: The highest upregulation was detected in IL-6 with 11 fold in T2D patients comparing with HCs. F/B ratio and gene expression levels were elevated in T2D patients. Firmicutes were positively correlated with studied gene expressions. A better understanding of the complex interaction between gut microbiota, environment, and diabetes will allow for more effective prevention and treatment strategies for T2D. © 2022 Verlag Klinisches Labor GmbH. All rights reserved. | en_US |
dc.description.sponsorship | Istanbul Üniversitesi: 3748, TSA-2016-3748 | en_US |
dc.description.sponsorship | We are grateful for the support of Istanbul University. This research was supported by the Scientific Research Fund of Istanbul University and Istanbul University - Cerrahpasa, Project and Grant No.: 3748 (TSA-2016-3748). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Verlag Klinisches Labor GmbH | en_US |
dc.relation.ispartof | Clinical Laboratory | en_US |
dc.identifier.doi | 10.7754/Clin.Lab.2022.211244 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Bacteroidetes | en_US |
dc.subject | Firmicutes | en_US |
dc.subject | Gut microbiota | en_US |
dc.subject | ILs | en_US |
dc.subject | TLRs | en_US |
dc.subject | Type 2 diabetes | en_US |
dc.subject | interleukin 1beta | en_US |
dc.subject | interleukin 6 | en_US |
dc.subject | interleukin 8 | en_US |
dc.subject | toll like receptor 2 | en_US |
dc.subject | toll like receptor 4 | en_US |
dc.subject | toll like receptor 5 | en_US |
dc.subject | interleukin 6 | en_US |
dc.subject | interleukin 8 | en_US |
dc.subject | RNA | en_US |
dc.subject | TLR2 protein, human | en_US |
dc.subject | TLR4 protein, human | en_US |
dc.subject | toll like receptor 2 | en_US |
dc.subject | toll like receptor 4 | en_US |
dc.subject | toll like receptor 5 | en_US |
dc.subject | adult | en_US |
dc.subject | aged | en_US |
dc.subject | Article | en_US |
dc.subject | Bacteroidetes | en_US |
dc.subject | blood sampling | en_US |
dc.subject | controlled study | en_US |
dc.subject | diabetic patient | en_US |
dc.subject | feces analysis | en_US |
dc.subject | female | en_US |
dc.subject | Firmicutes | en_US |
dc.subject | gene expression level | en_US |
dc.subject | human | en_US |
dc.subject | intestine flora | en_US |
dc.subject | major clinical study | en_US |
dc.subject | male | en_US |
dc.subject | non insulin dependent diabetes mellitus | en_US |
dc.subject | real time polymerase chain reaction | en_US |
dc.subject | upregulation | en_US |
dc.subject | Bacteroidetes | en_US |
dc.subject | Firmicutes | en_US |
dc.subject | gene expression | en_US |
dc.subject | genetics | en_US |
dc.subject | metabolism | en_US |
dc.subject | Adult | en_US |
dc.subject | Bacteroidetes | en_US |
dc.subject | Diabetes Mellitus, Type 2 | en_US |
dc.subject | Firmicutes | en_US |
dc.subject | Gastrointestinal Microbiome | en_US |
dc.subject | Gene Expression | en_US |
dc.subject | Humans | en_US |
dc.subject | Interleukin-6 | en_US |
dc.subject | Interleukin-8 | en_US |
dc.subject | RNA | en_US |
dc.subject | Toll-Like Receptor 2 | en_US |
dc.subject | Toll-Like Receptor 4 | en_US |
dc.subject | Toll-Like Receptor 5 | en_US |
dc.title | Firmicutes/Bacteroidetes Ratio in the Gut Microbiota and IL-1β, IL-6, IL-8, TLR2, TLR4, TLR5 Gene Expressions in Type 2 Diabetes | en_US |
dc.type | article | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.volume | 68 | en_US |
dc.identifier.startpage | 1903 | en_US |
dc.identifier.endpage | 1910 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | Bahar-Tokman, H., Istanbul University-Cerrahpasa, Cerrahpasa School of Medicine, Department of Medical Microbiology, Istanbul, Turkey; Demirci, M., Kirklareli University, School of Medicine, Department of Medical Microbiology, Kirklareli, Turkey; Keskin, F.E., Demiroglu Bilim University, School of Medicine, Department of Endocrinology, Istanbul, Turkey; Cagatay, P., Balikesir University, School of Medicine, Department of Biostatistics and Medical Informatic, Balikesir, Turkey; Taner, Z., Altinbas University, Altinbas School of Medicine, Department of Medical Microbiology, Istanbul, Turkey; Ozturk-Bakar, Y., Istanbul University-Cerrahpasa, Cerrahpasa School of Medicine, Department of Medical Microbiology, Istanbul, Turkey; Ozyazar, M., Yeni Yuzyil University, School of Medicine, Gaziosmanpasa Hospital, Department of Endocrinology, Istanbul, Turkey; Kiraz, N., Namik Kemal University, School of Medicine, Department of Medical Microbiology, Tekirdag, Turkey; Kocazeybek, B.S., Istanbul University-Cerrahpasa, Cerrahpasa School of Medicine, Department of Medical Microbiology, Istanbul, Turkey | en_US |
dc.identifier.pmid | 36125161 | en_US |
dc.identifier.scopus | 2-s2.0-85138143372 | en_US |
dc.authorscopusid | 57221970599 | |
dc.authorscopusid | 57220204043 | |
dc.authorscopusid | 56118245000 | |
dc.authorscopusid | 6507755715 | |
dc.authorscopusid | 57194164764 | |
dc.authorscopusid | 57211552167 | |
dc.authorscopusid | 6506930479 | |