dc.contributor.author | Ozgur, Burcin Aydin | |
dc.contributor.author | Cinar, Suzan Adin | |
dc.contributor.author | Coskunpinar, Ender | |
dc.contributor.author | Yilmaz, Abdullah | |
dc.contributor.author | Altunkanat, Derya | |
dc.contributor.author | Deniz, Gunnur | |
dc.contributor.author | Gurol, Ali Osman | |
dc.date.accessioned | 2024-02-04T13:29:38Z | |
dc.date.available | 2024-02-04T13:29:38Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0257-277X | |
dc.identifier.issn | 1559-0755 | |
dc.identifier.uri | https://doi.org/10.1007/s12026-022-09355-z | |
dc.identifier.uri | http://hdl.handle.net/11446/4704 | |
dc.description.abstract | Th cells play an important role in pathogenesis of type 1 diabetes (T1D). Peripheral blood mononuclear cells were isolated from peripheral blood samples from newly diagnosed (ND), 1-year (1YD), and 5-year T1D (5YD) patients (n:8 of each group), 8 healthy controls (HC), and cultured for 24 h under unstimulated (US) and stimulated conditions. Cell ratios of Th1, Th2, Th17, Treg, and intracellular levels of IFN-gamma, TNF-alpha, IL-10, TGF-beta, IL-5, IL-13, IL-17, and IL-21 cytokines were evaluated using the flow cytometry. mRNA expressions of transcription factors T-bet, GATA3, ROR-gamma t, and FOXP3 of these cells were determined by real-time PCR. Reduced CD4(+)CD25(high) cell ratios were detected in ND. CD4(+)CD25(high) cells were found to be reduced in ND and 1YD compared to HC under IL-2-stimulated conditions. Intracellular IFN-gamma and TNF-alpha levels were low in all patients under US and IL-12-stimulated conditions. IL-17A and IL-21 were found to be high in patients with IL-6-stimulated conditions. Expressions of IL-10 and TGF-beta have been observed to be reduced in patients. Th1/Th2, Th17/Treg, and Th1/Treg ratios were higher in patient groups. FOXP3 and GATA3 mRNA expressions were found to be low in patients, while ROR gamma t and T-bet mRNA levels were higher than HC. Th1, Th17, and Treg cells and their cytokines have been shown to be associated with type 1 diabetes. | en_US |
dc.description.sponsorship | Istanbul University Scientific Projects Coordination Unit; [56151] | en_US |
dc.description.sponsorship | This work was supported by the Istanbul University Scientific Projects Coordination Unit under Grant number 56151. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Springer | en_US |
dc.relation.ispartof | Immunologic Research | en_US |
dc.identifier.doi | 10.1007/s12026-022-09355-z | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Type 1 diabetes | en_US |
dc.subject | Th1 | en_US |
dc.subject | Th2 | en_US |
dc.subject | Th17 | en_US |
dc.subject | Treg | en_US |
dc.subject | T-bet | en_US |
dc.subject | GATA3 | en_US |
dc.subject | ROR-?t | en_US |
dc.subject | FOXP3 | en_US |
dc.subject | IFN-? | en_US |
dc.subject | TNF-a | en_US |
dc.subject | IL-10 | en_US |
dc.subject | TGF-(3 | en_US |
dc.subject | IL-5 | en_US |
dc.subject | IL-13 | en_US |
dc.subject | IL-17 | en_US |
dc.subject | IL-21 | en_US |
dc.title | The role of cytokines and T-bet, GATA3, ROR-?t, and FOXP3 transcription factors of T cell subsets in the natural clinical progression of Type 1 Diabetes | en_US |
dc.type | article | en_US |
dc.department | DBÜ | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.volume | 71 | en_US |
dc.identifier.startpage | 451 | en_US |
dc.identifier.endpage | 462 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department-temp | [Ozgur, Burcin Aydin] Demiroglu Bilim Univ, Fac Med, Dept Med Biol & Genet, Istanbul, Turkiye; [Ozgur, Burcin Aydin; Yilmaz, M. Temel] Demiroglu Bilim Univ, Diabet Applicat & Res Ctr, Istanbul, Turkiye; [Cinar, Suzan Adin; Yilmaz, Abdullah; Deniz, Gunnur; Gurol, Ali Osman] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Immunol, Istanbul, Turkiye; [Coskunpinar, Ender; Altunkanat, Derya] Hlth Sci Univ, Fac Med, Dept Med Biol, Istanbul, Turkiye; [Gurol, Ali Osman] Istanbul Univ, Diabet Applicat & Res Ctr, Istanbul, Turkiye; [Yilmaz, M. Temel] Demiroglu Bilim Univ, Fac Med, Dept Endocrinol & Metab, Istanbul, Turkiye | en_US |
dc.authorid | , Gunnur Deniz/0000-0002-0721-6213 | |
dc.authorid | cinar, suzan/0000-0002-8330-7010 | |
dc.identifier.pmid | 36595206 | en_US |
dc.identifier.scopus | 2-s2.0-85145506602 | en_US |
dc.identifier.wos | WOS:000907053600001 | en_US |
dc.authorwosid | , Gunnur Deniz/AAC-2909-2020 | |
dc.authorwosid | , G/JUF-3136-2023 | |
dc.authorwosid | cinar, suzan/J-4770-2019 | |