dc.description.abstract | Background. This study aimed to retrospectively investigate the correlation of mean Class I donor-specific antibody (DSA) intensity values detected in Luminex-based techniques with the results of complement-dependent cytotoxicity crossmatch (CDC-XM) and flow cytometry crossMethods. A total of 335 patients with kidney failure and their living donors whose CDC-XM, FC-XM, and single antigen based (SAB) tests were studied between 2018 and 2020 for transplant preparation from living donor candidates were included in the study. Patients were divided into 4 groups according to their mean fluorescence intensity (MFI) values of SAB assay.Results. Anti-HLA antibodies (class I and/or class II) were detected using SAB in 91.6% patients included in the study (MFI >1000). Class I DSA was positive in 34.8% of patients with anti-HLA antibodies. When CDC-XM and FC-XM results were evaluated in the 4 groups separated according to MFI values, 3 patients with DSA MFI <1000 had negative CDC-XM and T-B-FC-XM results. Of 32 patients with DSA-MFI between 1000 and 3000, 93.75% (n = 30) had T-B-FC-XM or CDC-XM-negative results, and 6.25% (n = 2) had B-FC-XM-positive results. The CDC-XM, T, and B-FC-XM were negative in all 17 patients with DSA-MFI between 3000 and 5000. Our results showed that MFI >5834 DSA values were significantly correlated with positive T-FC-XM (P < .001), and MFI >6016 values were significantly correlated with positive CDC-XM (P = .002). In addition, MFI values >5000 were associated with both CDC-XM and FC-XM in our study.Conclusions. The MFI values >5000 correlated with both CDC-XM and FC-XM. | en_US |
dc.department-temp | [Oguz, Suleyman Rustu] Demiroglu Bilim Univ, Dept Med Biol & Genet, Istanbul, Turkiye; [Oguz, Suleyman Rustu; Sinangil, Ayse; Barlas, Soykan; Ulusoy, Ezgi; Ogret, Yeliz; Ecder, Tevfik; Akin, Baris] Grp Florence Nightingale Hosp, Istanbul, Turkiye; [Oguz, Suleyman Rustu; Ulusoy, Ezgi] Demiroglu Bilim Univ, Tissue Typing Lab, Istanbul, Turkiye; [Sinangil, Ayse; Ecder, Tevfik] Demiroglu Bilim Univ, Dept Nephrol, Istanbul, Turkiye; [Barlas, Soykan; Ogret, Yeliz; Akin, Baris] Demiroglu Bilim Univ, Dept Gen Surg, Istanbul, Turkiye; [Ciftci, Hayriye Senturk; Izgi, Demet Kivanc] Istanbul Univ, Istanbul Fac Med, Dept Med Biol, Istanbul, Turkiye; [Izgi, Demet Kivanc] Istanbul Univ, Inst Hlth Sci, Dept Med Biol, Istanbul, Turkiye | en_US |