The combined ameliorative effects of ?-lipoic acid, selenium, and vitamin E on the livers of STZ-diabetic mice

Abstract

Background and Aims: The aim of this study was to investigate the histological and biochemical effects of the antioxidant combination on liver tissue of streptozotocin (STZ)-induced diabetic mice.Methods: Five groups of mice were given a citrate buffer (CB), the antioxidant solvents (AS), the antioxidant combination (A) (alpha-lipoic acid, selenium, and vitamin E), STZ (D), the antioxidant combination and STZ (A+D). The mice were sacrificed, and their liver tissues were taken out. The liver tissues were examined histologically and immune+ cell numbers of can-nabinoid receptors (CB1R and CB2R) were detected. Xanthine oxidase (XO) activity, glutathione (GSH) and lipid peroxidation (LPO) levels, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), gamma-glutamyl transferase (GGT), paraoxonase (PON), glucose-6-phosphate dehydrogenase (G6PD) activities, protein carbonyl content (PCC) and advanced oxidation protein product (AOPP), sialic acid, fucose, hexose and hydroxyproline (OH-proline) levels were biochemically determined.Results: Certain degenerative changes were reduced in the A+D group compared histologically to the D group. There were no significant changes in the number of CB1R immune+ cells. The number of CB2R immune+ cells was significantly reduced in the D group compared to the CB group. The GSH level, CAT, SOD, GR, GPx, GST, PON, and G6PD activities were increased while XO and GGT activity, LPO, PCC, AOPP, hexose, fucose, sialic acid, and OH-proline level were biochemically decreased in the A+D group compared to the D group.Conclusion: The use of the antioxidant combination had a positive effect on the livers of diabetic mice with histochemical and biochemical changes, while there was no effect on the regulation of cannabinoid receptors expressions.