Research Article Nasal Oxytocin's Anticonvulsant Effects on Pentylenetetrazolinduced Convulsions in Rats

Abstract

Background and Objective: Neurodegenerative proteins and pro-inflammatory mechanisms may cause epilepsy through excitatory-inhibitory imbalance. Drug resistance is common, therefore this disease requires affordable, accessible therapy. High-dose intraperitoneal oxytocin (OT) reduced seizures in experiments. Drug delivery is easy through the nasal mucosa. This study goal was to explain the anti-convulsant properties of nasal OT and its mechanism in rat seizures caused by pentylenetetrazol (PTZ). Materials and Methods: As, 36 male adult Sprague-Dawley rats were randomly assigned to 1 of 2 groups: Group A was utilized for electroencephalograms (EEG), whereas, group B was assigned to cognitive and behavioural evaluations. Of the thirty six rats, eighteen were used for behavioral investigations and the other eighteen were used for EEG recordings. Following the administration of OT, 35 mg kgG1 PTZ was used to capture the EEG. For 10 days, 40 g/kg/day of OT was administered orally, followed by the injection of PTZ about 70 mg kgG1 dose for behavioral investigations. In order to record the EEG, the reference electrode and the dura was implanted with electrodes above the left side of cortex of frontal section and cerebellum, respectively. The racine convulsion scale scores, percentage of spike in EEG, first myoclonic jerk beginning moment and levels of IL-1 beta, GAD-67, TNF-alpha and MDA in brain specimens were all compared across groups. Results: In comparison to the saline group, the first myoclonic jerk beginning time was noticeably faster in the group of OT (p<0.05). The convulsion scale used by Racine did not alter appreciably. In comparison to the saline group, the OT group had decreased spike percentages (p<0.05). The OT group's TNF-alpha, MDA and IL-1 beta levels were found to be considerably lower than those of the saline group (p<0.01, p<0.001 and p<0.001). In OT group's, GAD-67 increased considerably (p<0.001). Conclusion: Findings of this study show that OT has inhibitory actions against PTZ-triggered seizures as well as against the oxidative and inflammatory harm caused by PTZ.