Association of Different Doses of Curcumin with Preadipocyte to Adipocyte Differentiation

Abstract

Objective: Human adipose tissue participates in fat storage and immune response. Curcumin (CUR) decreases adipocyte differentiation by inhibiting inflammatory cytokines and by activating anti-inflammatory cytokines. In this study, we aimed to determine the suppressor effects of different doses of CUR (0.5 µM, 5 µM, 10 µM, 20 µM, 50 µM) on preadipocyte-adipocyte differentiation and its anti-inflammatory role in adipocytes. Methods: Differentiation of cells was performed using Oil Red O. The mRNA expression levels of adiponectin, C/EBPα, COX-2, IL-6, leptin, NFκB1, PPARγ, SIRT-1, TNF-α, TRPV1, UCP2, VEGF-A, VEGF-RI, and VEGF-RII were evaluated in preadipocyte and adipocytes. Results: CUR decreased the differentiation of preadipocytes-adipocytes and the release of proinflammatory cytokines by regulating the expression of C/EBPα and PPARγ gene expressions. Conclusion: CUR inhibited adipogenic transcription factors and adipocyte differentiation at all concentrations. The anti-inflammatory effect was greatest at 50 µM. © 2025 Elsevier B.V., All rights reserved.